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Proteins, Expression, Isolation and Analysis> | … The following primary antibodies were used: MAP-2 (rabbit, 1:400, Millipore, Billerica, MA, USA: AB5622), MAP-2 (chicken, 1:4000, Novus Biologicals, Littleton, CO, USA: NB300-213), β III -tubulin (rabbit, 1:2000, GenScript, Piscataway, NJ, USA: A01627), β III -tubulin (chicken, 1 … | Get A Quote |
Long-term neural differentiation of human pluripotent stem cells (hPSCs) is associated with enhanced neuronal maturation, which is a necessity for creation of representative in vitro models. It also induces neurogenic-to-gliogenic fate switch, increasing proportion of endogenous astrocytes formed from the common neural progenitors. However, the significance of prolonged differentiation on the neural cell type composition and functional development of hPSC-derived neuronal cells has not been well characterized. Here, we studied two hPSC lines, both of which initially showed good neuronal differentiation capacity. However, the propensity for endogenous astrogenesis and maturation state after extended ... More